Category: Biology
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The Genetic Code
Goals Explain how the mRNA sequence encodes amino acids Describe what is meant by a reading frame and why it is important Using a codon table, translate a sequence of DNA/RNA into amino acids Protein Structure on Function The structure of the protein dictates its function. Thats because most of the functions done in the…
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Protein Translation
Goals Outline the process of translation Describe the structure and role of tRNAs Explain and contrast the process of translation initiation in eukaryotes and prokaryotes Describe the role of the A,P, and E sites in the ribosome during translation elongation Explain how release factor terminates translation Explain how the relationship between transcription and translation differs…
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Protein Techniques
Goals: Outline SDS-PAGE, Western blot and Immunofluorescence experiments and apply them to relevant examples Apply the above mentioned techniques to experimental questions (and know why to use each one) Probe Hybridization A probe is a complementary to the DNA/RNA of sequence. This probe is a labeled complementary strand of DNA or RNA, and can specifically…