The lac Operon Explained

The lac operon is the gene which is incorporated in the metabolism of glucose. The lactose metabolism is regulated my the expression and non-expression of the lac operon. The gene which is transcribed contains the CAP ( the region where the activator binds), and then there is the lacI gene, which independently expresses the repressor…

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Regulation of Gene Expression and Operons Explained

Goals Explain why it is important for cells to express different genes at different times, in prokaryotes and eukaryotes. Describe the general function of a transcriptional activator and transcriptional repressor Describe what an operon is and its basic regulation Explain how and under what conditions the lux operon is regulated Predict the effect of mutations…

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Are GMO’s Bad?

What are GMO’s What are GMO’s? Do you know what they are? Credit: Jimmy Kimmel Live The acronym of GMO’s is Genetically Modified Organisms. GMO’s when not talked about food is known as a Transgenic organism, meaning genes of one organism is transferred into another. They contain DNA from another species. This is done in…

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Transcription in Eukaryotes Explained

Goals Describe the major differences (polymerases, mechanism of initiation, promoter, termination) between eukaryotic and prokaryotic transcription Describe the structure and function of the 5′ cap and polyA tail of an mRNA Transcription Overview An enzyme called RNA polymerase is used on a template DNA to create a new strand of RNA. The key steps to…

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RNA Techniques

Goals Explain the function of a nucleic acid probe and describe the features that are required for its function. Outline the major steps in Northern blotting, RT-PCR, qPCR, microarray analysis and in situ hybridization. Explain the purpose of these techniques. Apply the above mentioned techniques to experimental questions (and know why to use each one)…

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