Goals Explain the function of a nucleic acid probe and describe the features that are required for its function.Outline the major steps in Northern blotting, RT-PCR, qPCR, microarray analysis and in situ hybridization. Explain the purpose of these techniques.Apply the above mentioned techniques to experimental questions (and know why to use each one) Measuring Gene …
The Genetic Code
Goals Explain how the mRNA sequence encodes amino acidsDescribe what is meant by a reading frame and why it is importantUsing a codon table, translate a sequence of DNA/RNA into amino acids Protein Structure on Function The structure of the protein dictates its function. Thats because most of the functions done in the cell are …
Protein Translation
Goals Outline the process of translationDescribe the structure and role of tRNAsExplain and contrast the process of translation initiation in eukaryotes and prokaryotesDescribe the role of the A,P, and E sites in the ribosome during translation elongationExplain how release factor terminates translationExplain how the relationship between transcription and translation differs between eukaryotes and prokaryotes Overview …