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Goals Explain why it is important for cells to express different genes at different times, in prokaryotes and eukaryotes.Describe the general function of a transcriptional activator and transcriptional repressorDescribe what an operon is and its basic regulationExplain how and under what conditions the lux operon is regulatedPredict the effect of mutations in the lux operon …
Continue reading "Regulation of Gene Expression and Operons Explained"
Goals Describe the major differences (polymerases, mechanism of initiation, promoter, termination) between eukaryotic and prokaryotic transcriptionDescribe the structure and function of the 5' cap and polyA tail of an mRNA Transcription Overview An enzyme called RNA polymerase is used on a template DNA to create a new strand of RNA. The key steps to this …
Goals Describe the structure and function of the 5' cap and polyA tail of an mRNAExplain the mechanism of splicing and how it is enhanced by the spliceosomeExplain how and why transcription and mRNA processing are coupledDraw an RNApolII transcript including relevant structural features (cap, polyA tail, introns, exons)Explain the role of mRNA processing in …
Goals Explain the function of a nucleic acid probe and describe the features that are required for its function.Outline the major steps in Northern blotting, RT-PCR, qPCR, microarray analysis and in situ hybridization. Explain the purpose of these techniques.Apply the above mentioned techniques to experimental questions (and know why to use each one) Measuring Gene …
Goals Outline the process of translationDescribe the structure and role of tRNAsExplain and contrast the process of translation initiation in eukaryotes and prokaryotesDescribe the role of the A,P, and E sites in the ribosome during translation elongationExplain how release factor terminates translationExplain how the relationship between transcription and translation differs between eukaryotes and prokaryotes Overview …