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Goals Explain why it is important for cells to express different genes at different times, in prokaryotes and eukaryotes.Describe the general function of a transcriptional activator and transcriptional repressorDescribe what an operon is and its basic regulationExplain how and under what conditions the lux operon is regulatedPredict the effect of mutations in the lux operon …
Continue reading "Regulation of Gene Expression and Operons Explained"
Goals Describe the structure and function of the 5' cap and polyA tail of an mRNAExplain the mechanism of splicing and how it is enhanced by the spliceosomeExplain how and why transcription and mRNA processing are coupledDraw an RNApolII transcript including relevant structural features (cap, polyA tail, introns, exons)Explain the role of mRNA processing in …
Goals Explain the function of a nucleic acid probe and describe the features that are required for its function.Outline the major steps in Northern blotting, RT-PCR, qPCR, microarray analysis and in situ hybridization. Explain the purpose of these techniques.Apply the above mentioned techniques to experimental questions (and know why to use each one) Measuring Gene …
Goals Explain how the mRNA sequence encodes amino acidsDescribe what is meant by a reading frame and why it is importantUsing a codon table, translate a sequence of DNA/RNA into amino acids Protein Structure on Function The structure of the protein dictates its function. Thats because most of the functions done in the cell are …
Goals: Outline SDS-PAGE, Western blot and Immunofluorescence experiments and apply them to relevant examples Apply the above mentioned techniques to experimental questions (and know why to use each one) Probe Hybridization A probe is a complementary to the DNA/RNA of sequence. This probe is a labeled complementary strand of DNA or RNA, and can specifically …